The Development of Mucosal Immunization as an Alternative Approach for Production of Antisera Against Saw Scale Viper from Sindh


 The reported cases of snake bite are 5.4 million per year, making the serious envenoming issue so, that WHO categorises in neglected tropical diseases. Anti venom is produced by intramuscular injection crude with different formulation adjuvants to equine animals, but these injections have several health impact equine. Snake species categorised according geographical locations, and there a composition at locations. So, this research had been designed evaluated new oral adjuvant for viper family Sindh, Pakistan. This animal immune based analysis consisting two Saw scale from Thar costal area Sindh because both habitat morphological appearance. toxin protein, combined mineral oil immunization. via route specific IgG horse give maximum response three doses venom. antibody titer was measured ELISA showed significant results groups. For confirmation antibodies isolated serum, serum neutalization assay immunodiffusion test were carried out. immunization, safe, efficient time saving production Immunoglobulins IgG.